The application of transmitted light for cell counting
This system can also add transmission light imaging and color imaging functions on the basis of 4-color fluorescence channel imaging, a variety of magnification objectives are optional, environmental control system (EC), injector system, etc. The application of transmitted light for cell counting and apoptosis detection and analysis software can divide and count cells without resorting to any dye molecules. Autophagy dysfunction and mitochondrial integrity are related to the pathogenesis of various diseases, including neurodegenerative diseases and cardiovascular diseases.
The instrument based on cytology research can use ImageXpress;Pico automatic cell imaging system combined with CellReporter-XpressTM automatic image analysis software. Evaluation of cell morphology and viability Imaging and analysis methods as an effective tool can provide information on a variety of parameters, including cell viability, characteristics of cells at different stages, cell adhesion and distribution status, cytoskeletal integrity (morphology) and mitochondrial potential Change etc. In order to observe the actin cytoskeleton, the cells were fixed with 4% paraformaldehyde (Sigma) and stained with phalloidin linked to AlexaFluor; 488 (AF488).
Multiple parameters including cell viability, apoptosis, mitochondrial membrane potential, and autophagy are of great significance for the study of the toxicity mechanism of living or fixed cells.Poor water quality in the first-grade effluent ①. Insufficient water supply, improve water supply or replace pretreatment filter element. Replace the high-pressure protection switch. At this time, the full water indicator light will be on.
The cryo rack does not make water ①. ②. ③. Replace the low-voltage protection switch. Short-circuiting may cause the booster pump to idle and damage the pump. The system function is abnormal: the system circuit may be damaged, replace the circuit board. Open the pure bucket ball valve. invited a technician, Master Su, to explain in detail the common troubleshooting of the ultra-pure water machine in the laboratory! 1. The circulation pump may be damaged, and the circulation treatment cannot be performed. The amount of water produced by the system is too small. It may be deposited for too long, and drain it., Ltd
