The point of observation is to describe in detail the condition
The mother rat has Conical Centrifuge Tube Suppliers all the hairs of one of the young rats. Animal scratching caused by parasites or other skin infections may also cause localized hair loss or stress. In your daily work, you have many opportunities to observe the normal behavior and behavior of animals. (Photo courtesy of Dr. Causes When animals of the wrong population or strain enter a certain group and provide genetic material, genetic contamination may occur. The point of observation is to describe in detail the condition and extent of the change in coat color.
This mouse is male DBA/2. Figure 2D Another animal with over-grooming. Sneezing, dysentery or changes in appetite also belong to the category of clinical symptoms, and we can also call these abnormal changes as abnormal findings. Notice the white hair on the black workbench. In order to thank many customers in the life science industry for their long-term support, starting from January 2016, Vitton Lihua has exclusively issued a limited Chinese edition in mainland China, offering you valuable experimental reference materials. It has spontaneous mutations, resulting in a normal-colored back and pale abdomen.
This issue mainly introduces you to some of the clinical symptoms of hair in experimental rodents. If an animal's coat color changes in the entire litter, it may also be caused by a spontaneous genetic mutation. Figure 2A These pictures (Figures 2A-2E) show the different types of shaving. Figure 2C This group of animals has different degrees of shaving. Four of its six offspring exhibited this mutation. § More reference content The above is just the 'tip of the iceberg' of Charles River Laboratories' monograph 'Handbook of Clinical Signs in Rodents and Rabbits'.
This confounding result may not be observed after several generations. In this case, all animals in the cage may be affected (see missing hair). In addition, the regrowth of hair after an animal injury may be different from before. Description of changes in coat color Compared with normal animals of the same sex, age or strain, the color of the whole or part of the animal's hair has changed. If you need the book, you can consult your regional manager to obtain the internal publication. Garner) Figure 2E The female rat has plucked all the hairs on the abdomen and legs, perhaps because of shaving, or because of the need to build a nest for the young rats. Figure 3 This figure shows the normal hair loss that occurs when the guinea pig is picked up.
Distiller is an appliance that uses distillation to separate substances
The power Long Pipette tip socket of the refrigerator is 13A. 8. Before use or maintenance, please formulate operating procedures and operate according to the procedures. Generally, 13A sockets can meet the requirements. The glassware washing machine is mainly used for cleaning glassware, beakers, test tubes, slides, coverslips, glass pipettes, pipettes and other laboratory utensils in the laboratory. Freeze dryer is a machine that freezes the water-containing material into a solid state, and then sublimates the water from the solid state to a gaseous state to remove the water and preserve the material.
The power requirement is 30A. 5. 1. Other commonly used equipment such as dryers, autoclaves, biological safety cabinets, clean benches, electron microscopes, nuclear magnetic resonance imaging devices, fire extinguishers, etc. 3. 9. 2. 10. Drying box BO-200N drying box BO-30F blast drying box BV-20 vacuum drying box is divided into two categories: electric heating blast drying box and vacuum drying box.
Distiller is an appliance that uses distillation to separate substances. Generally used to dry clean items. 4. Low-temperature refrigerators are generally divided into -40 degrees, -90 degrees, -110 degrees, -130 degrees, and -160 degrees.This article mainly explains the basic knowledge of laboratory instruments, to understand what are the commonly used instruments in conventional laboratories. There are two types: filtration type and sedimentation type. 11.
The ice maker is a kind of refrigeration mechanical equipment that produces ice after the water passes through the evaporator and is cooled by the refrigeration system refrigerant.. Constant temperature incubator BI-200F constant temperature incubator is divided into table type and floor type. 7. Sub-table and floor-standing. The power socket of the vertical refrigerator is 13A, and the power socket of the horizontal refrigerator is 20A. Centrifuge 800 electric centrifuge uses centrifugal force to accelerate the separation of different materials that need to be separated. 6. The scintillation counter uses rays or particles to cause scintillators to emit light and record the intensity and energy of the rays through photoelectric devices. 13A socket and requires oxygen or carbon dioxide
The accessories can make the actual operation easier
For example, the BV240A model of Sansite can use foot switch and hand-held manipulator to control liquid suction. The form of the waste liquid bottle and the host: the basic laboratory waste liquid suction device host and waste liquid One bottle is only connected by a vacuum pump tube, which is relatively simple in structure and highly cost-effective; most models have a fixed bracket on the side of the host, and the waste liquid bottle can be loaded into the bracket. Serological Pipette Company Configuration of other accessories:
The accessories can make the actual operation easier: for example, the vacuum gauge and the vacuum regulating valve can observe the working status of the machine during the working process, and then adjust the suction speed according to the actual needs; water blocking filter The device can block the liquid 100%, completely solving the problem of waste liquid being sucked into the pump. 2. The configuration of the waste liquid bottle: the capacity and quantity of the waste liquid bottle will determine the total amount of waste liquid. For more product consultation and usage introduction of cell culture waste liquid suction pump, please refer to our website. 5. This design has obvious advantages and is convenient
When the whole machine moves, the waste liquid bottle is not easy to dump; the most advanced design at present is an integrated model, that is, there is a fixed seat in the middle of the body, and the waste liquid bottle is directly put into the fixed seat, except for the convenience of moving and the waste liquid bottle. Vacuum degree and suction rate: these are the core parameters of this instrument.
At this time, if the machine is still controlled to start and stop through the power switch on the host panel, the gloves will be contaminated.The cell waste liquid suction vacuum pump is a basic equipment that a biological laboratory must use in a clean room. The waste bottle on the side close to the vacuum pump can be used to hold waste liquid or as a buffer bottle to prevent back suction. Customers often have no way to start with many models when choosing.
The substrate catalyzed by HRP is hydrogen peroxide
First, the laboratory cryo rack, equipment and hardware facilities must be in good condition, and the reagents and measurement methods must be followed. For the sample absorbance value ( A) If it is higher than the CO value, it is judged as positive, and on the contrary, it is judged as negative. It is prepared before use.
4 Judgment of results and report 4. In my actual work, the author found that patients whose results are near the CO value have poor repeatability and are also likely to cause medical disputes. Therefore, it is necessary to select and order long batches of reagents and ensure storage conditions. In summary, the ELISA detection operation steps are complex, and many factors that may affect the measurement result are distributed in each step of the measurement operation. The substrate catalyzed by HRP is hydrogen peroxide.
Serum samples should be centrifuged sufficiently, otherwise false positive results may be caused by non-specific adsorption of fibrinogen in the micropores. If all kinds of acidic terminating liquids are selected, the blue color will be changed to yellow. The ELISA plate can be placed in a water bath box, and the bottom of the plate should be close to the water surface to quickly balance the temperature.
The wet box should use materials with good heat transfer properties (such as metal, etc. In order to ensure the stability of the results, it is necessary to strictly follow the temperature and time specified in the kit instructions, and the temperature and time cannot be changed at will. The hospital strictly implemented the review system in actual work and achieved good results. Therefore, the establishment of a review system and increased review efforts can ensure the accuracy of the results, such as Re-examine the test results of the 'two-and-a-half' hepatitis B in the 'gray zone' and the rare mode.
The balance error of the centrifuge tube should be within
During the centrifugation process, the operator must not leave the centrifuge room. After use, you must register and indicate the use. It will also be a safety hazard. After tightening the rotor cover, be sure to touch your finger to see that there is no gap between the rotor and the cover. 5. 1. And wipe the remaining water in the cavity, the centrifuge cover should be opened. This process cannot be ignored because of the hurry.
When the rotor is pre-cooled, the rotor cover can be placed on the platform of the centrifuge, or placed on the experimental table, and it must not be tightened and floated on the rotor. 2. OK key to confirm. c) Select the rotor setting item, then use the plus and minus keys to set the rotor type, and press the OK key to confirm. Generally, unplug the power cord after shutdown. four. Once the centrifuge is found to be abnormal (such as imbalance causing the machine to vibrate significantly, or noisy), you should Immediately press the stop button to stop centrifugation. When the centrifuge is in the pre-cooling state, pre-cooling is very necessary. For more information
During the centrifugation process, the operator should not leave the centrifuge room. Machine failures occur during the use of the instrument, and you should contact us in time when the parts are damaged., Ltd. 4. The balanced centrifuge tube should be placed symmetrically in the rotor, and then the rotor cover should be closed and the screws should be tightened. Liquid residue, keep cryo tubes the bricks of the centrifuge dry. If it exceeds, it will cause the liquid to overflow easily. 9. If there is a gap, unscrew and re-tighten the centrifuge until the seamless gap is confirmed. 6.
During ultracentrifugation, the liquid must be filled with the centrifuge tube, because vacuum is required during ultracentrifugation, so the deformation of the centrifuge tube must be avoided only when it is full. When the centrifuge is in the pre-cooled state, the centrifuge cover must be closed. In case of poor sealing of the centrifuge tube cover, the liquid cannot be filled up to prevent overflow, which will affect the normal operation of the sensor. Therefore, when using a centrifuge, you must pay attention to the following operations. Select the centrifugal parameters: a) Select the temperature setting item, and then use the plus and minus buttons to set the centrifugal temperature, and the OK button to confirm. The balance error of the centrifuge tube should be within 0.
The application of transmitted light for cell counting
This system can also add transmission light imaging and color imaging functions on the basis of 4-color fluorescence channel imaging, a variety of magnification objectives are optional, environmental control system (EC), injector system, etc. The application of transmitted light for cell counting and apoptosis detection and analysis software can divide and count cells without resorting to any dye molecules. Autophagy dysfunction and mitochondrial integrity are related to the pathogenesis of various diseases, including neurodegenerative diseases and cardiovascular diseases.
The instrument based on cytology research can use ImageXpress;Pico automatic cell imaging system combined with CellReporter-XpressTM automatic image analysis software. Evaluation of cell morphology and viability Imaging and analysis methods as an effective tool can provide information on a variety of parameters, including cell viability, characteristics of cells at different stages, cell adhesion and distribution status, cytoskeletal integrity (morphology) and mitochondrial potential Change etc. In order to observe the actin cytoskeleton, the cells were fixed with 4% paraformaldehyde (Sigma) and stained with phalloidin linked to AlexaFluor; 488 (AF488).
Multiple parameters including cell viability, apoptosis, mitochondrial membrane potential, and autophagy are of great significance for the study of the toxicity mechanism of living or fixed cells.Poor water quality in the first-grade effluent ①. Insufficient water supply, improve water supply or replace pretreatment filter element. Replace the high-pressure protection switch. At this time, the full water indicator light will be on.
The cryo rack does not make water ①. ②. ③. Replace the low-voltage protection switch. Short-circuiting may cause the booster pump to idle and damage the pump. The system function is abnormal: the system circuit may be damaged, replace the circuit board. Open the pure bucket ball valve. invited a technician, Master Su, to explain in detail the common troubleshooting of the ultra-pure water machine in the laboratory! 1. The circulation pump may be damaged, and the circulation treatment cannot be performed. The amount of water produced by the system is too small. It may be deposited for too long, and drain it., Ltd
