The substrate catalyzed by HRP is hydrogen peroxide
First, the laboratory cryo rack, equipment and hardware facilities must be in good condition, and the reagents and measurement methods must be followed. For the sample absorbance value ( A) If it is higher than the CO value, it is judged as positive, and on the contrary, it is judged as negative. It is prepared before use.
4 Judgment of results and report 4. In my actual work, the author found that patients whose results are near the CO value have poor repeatability and are also likely to cause medical disputes. Therefore, it is necessary to select and order long batches of reagents and ensure storage conditions. In summary, the ELISA detection operation steps are complex, and many factors that may affect the measurement result are distributed in each step of the measurement operation. The substrate catalyzed by HRP is hydrogen peroxide.
Serum samples should be centrifuged sufficiently, otherwise false positive results may be caused by non-specific adsorption of fibrinogen in the micropores. If all kinds of acidic terminating liquids are selected, the blue color will be changed to yellow. The ELISA plate can be placed in a water bath box, and the bottom of the plate should be close to the water surface to quickly balance the temperature.
The wet box should use materials with good heat transfer properties (such as metal, etc. In order to ensure the stability of the results, it is necessary to strictly follow the temperature and time specified in the kit instructions, and the temperature and time cannot be changed at will. The hospital strictly implemented the review system in actual work and achieved good results. Therefore, the establishment of a review system and increased review efforts can ensure the accuracy of the results, such as Re-examine the test results of the 'two-and-a-half' hepatitis B in the 'gray zone' and the rare mode.
